› 5g35

X-ray diffraction
2Å resolution

Structure of Rad14 in complex with acetylaminopyren-C8-guanine containing DNA

Released:
DOI: 10.2210/pdb5g35/pdb
PDB entry 5g35 coloured by chain, front view.
PDB entry 5g35 coloured by chain, side view.
PDB entry 5g35 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for Bulky-Adduct DNA-Lesion Recognition by the Nucleotide Excision Repair Protein Rad14.
Simon N, Ebert C, Schneider S
Chemistry 22 10782-5 (2016)
PMID: 27223336

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-509447 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA repair protein RAD14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 15.61 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28519 (Residues: 188-306; Coverage: 32%)
Gene names: RAD14, YM8325.02C, YMR201C
Sequence domains: XPA protein C-terminus
5'-D(*GP*CP*TP*CP*TP*AP*8PYP*TP*CP*AP*TP*CP*AP*CP)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 15 nucleotides
Theoretical weight: 4.76 KDa
Source organism: synthetic construct
5'-D(*GP*TP*GP*AP*TP*GP*AP*CP*GP*TP*AP*GP*AP*GP)-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 14 nucleotides
Theoretical weight: 4.38 KDa
Source organism: synthetic construct

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand 8PY 2 x 8PY

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41
Unit cell:
a: 53.17Å b: 53.17Å c: 131.307Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.254
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

The XPA Protein-Life under Precise Control.
Krasikova et al. (2022)
5 other articles

1 mention without citation

Structural Connection between Activation Microswitch and Allosteric Sodium Site in GPCR Signaling.
White et al. (2018)