› 5g3l

X-ray diffraction
1.72Å resolution

ESCHERICHIA COLI HEAT LABILE ENTEROTOXIN TYPE IIB B-PENTAMER COMPLEXED WITH SIALYLATED SUGAR

Released:
DOI: 10.2210/pdb5g3l/pdb
PDB entry 5g3l coloured by chain, front view.
PDB entry 5g3l coloured by chain, side view.
PDB entry 5g3l coloured by chain, top view.
Source organism: Escherichia coli HB101
Primary publication:
Biochemical and structural characterization of the novel sialic acid-binding site of Escherichia coli heat-labile enterotoxin LT-IIb.
Zalem D, Ribeiro JP, Varrot A, Lebens M, Imberty A, Teneberg S
Biochem J 473 3923-3936 (2016)
PMID: 27562297

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-545460 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Heat-labile enterotoxin IIB, B chain Chains: D, E, H
Molecule details ›
Chains: D, E, H
Length: 99 amino acids
Theoretical weight: 10.79 KDa
Source organism: Escherichia coli HB101
Expression system: Escherichia coli
UniProt:
  • Canonical: P43529 (Residues: 24-122; Coverage: 100%)
Sequence domains: Type II heat-labile enterotoxin , B subunit (LT-IIB)
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Heat-labile enterotoxin IIB, B chain Chains: F, G
Molecule details ›
Chains: F, G
Length: 99 amino acids
Theoretical weight: 10.78 KDa
Source organism: Escherichia coli HB101
Expression system: Escherichia coli
UniProt:
  • Canonical: P43529 (Residues: 24-122; Coverage: 100%)
Sequence domains: Type II heat-labile enterotoxin , B subunit (LT-IIB)
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: SIA, GAL
Carbohydrate polymer
2 bound ligands:
Ligand NA 4 x NA
Ligand SIA 2 x SIA
1 modified residue:
Ligand ME0 3 x ME0

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 48.564Å b: 68.015Å c: 154.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.197
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Protein Toxins That Utilize Gangliosides as Host Receptors.
Zuverink et al. (2018)
2 more

1 mention without citation

Characterization of the ganglioside recognition profile of Escherichia coli heat-labile enterotoxin LT-IIc.
Zalem et al. (2022)