› 5g4q

X-ray diffraction
2.3Å resolution

H.pylori Beta clamp in complex with 5-chloroisatin

Released:
DOI: 10.2210/pdb5g4q/pdb
PDB entry 5g4q coloured by chain, front view.
PDB entry 5g4q coloured by chain, side view.
PDB entry 5g4q coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Screening of E. coli β-clamp Inhibitors Revealed that Few Inhibit Helicobacter pylori More Effectively: Structural and Functional Characterization.
Pandey P, Verma V, Dhar SK, Gourinath S
OpenAccess logo Antibiotics (Basel) 7 (2018)
PMID: 29324718

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-518821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 42.24 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O25242 (Residues: 1-374; Coverage: 100%)
Gene names: HP_0500, dnaN
Sequence domains:
Structure domains: DNA Polymerase III, subunit A, domain 2

Ligands and Environments

1 bound ligand:
Ligand 2HQ 1 x 2HQ
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 82.311Å b: 66.372Å c: 89.119Å
α: 90° β: 115.96° γ: 90°
R-values:
R R work R free
0.23 0.227 0.285
Expression system: Escherichia coli BL21

Quick links

Citations

2 mentions without citation

Potential of rescue and reactivation of tumor suppressor p53 for cancer therapy.
Hibino et al. (2022)
1 more