5g62

X-ray diffraction
1.99Å resolution

S.pneumoniae ABC-transporter substrate binding protein FusA EF-hand mutant in complex with fructo-nystose

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-101466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Fructooligosaccharide ABC transporter substrate-binding protein FusA Chains: A, B
Molecule details ›
Chains: A, B
Length: 494 amino acids
Theoretical weight: 56.25 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2URD6 (Residues: 47-538; Coverage: 95%)
Gene names: SP_1796, fusA

Ligands and Environments

Carbohydrate polymer : NEW Components: ZEE, UEA, FRU
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 122.58Å b: 122.58Å c: 169.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.208
Expression system: Escherichia coli BL21(DE3)