› 5ggw

X-ray diffraction
1.76Å resolution

Crystal structure of Class C beta-lactamase

Released:
DOI: 10.2210/pdb5ggw/pdb
PDB entry 5ggw coloured by chain, front view.
PDB entry 5ggw coloured by chain, side view.
PDB entry 5ggw coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for the extended substrate spectrum of AmpC BER and structure-guided discovery of the inhibition activity of citrate against the class C β-lactamases AmpC BER and CMY-10.
Na JH, Cha SS
Acta Crystallogr D Struct Biol 72 976-85 (2016)
PMID: 27487828

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 40.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7SNP8 (Residues: 21-377; Coverage: 100%)
Gene names: C6669_06645, CCS08_01890, CR538_23215, DS732_02540, FJQ40_01745, FJQ40_28765, NCTC8959_04306, NY836_19450, ampC, blaEC
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2221
Unit cell:
a: 83.732Å b: 182.088Å c: 141.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.23 0.259
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Class C β-Lactamases: Molecular Characteristics.
Philippon et al. (2022)
4 other articles

3 mentions without citation

Crystal structure of AmpC BER and molecular docking lead to the discovery of broad inhibition activities of halisulfates against β-lactamases.
Jeong et al. (2021)
2 more