› 5gjh

X-ray diffraction
1.2Å resolution

Gads SH2 domain/CD28-derived peptide complex

Released:
DOI: 10.2210/pdb5gjh/pdb
PDB entry 5gjh coloured by chain, front view.
PDB entry 5gjh coloured by chain, side view.
PDB entry 5gjh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structures and Thermodynamic Analysis Reveal Distinct Mechanisms of CD28 Phosphopeptide Binding to the Src Homology 2 (SH2) Domains of Three Adaptor Proteins.
Inaba S, Numoto N, Ogawa S, Morii H, Ikura T, Abe R, Ito N, Oda M
J Biol Chem 292 1052-1060 (2017)
PMID: 27927989

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131119 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GRB2-related adapter protein 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75791 (Residues: 58-155; Coverage: 30%)
Gene names: GADS, GRAP2, GRB2L, GRID
Sequence domains: SH2 domain
Structure domains: SH2 domain
T-cell-specific surface glycoprotein CD28 Chains: B, D
Molecule details ›
Chains: B, D
Length: 8 amino acids
Theoretical weight: 1.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10747 (Residues: 189-196; Coverage: 4%)
Gene name: CD28

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 33.602Å b: 53.338Å c: 108.302Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.17
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Recent Advances in Allogeneic CAR-T Cells.
Kim et al. (2020)
1 more

4 mentions without citation

A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription.
Sdano et al. (2017)
3 more