› 5gkk

X-ray diffraction
2Å resolution

Crystal structure of a homing endonuclease, I-TnaI

Released:
DOI: 10.2210/pdb5gkk/pdb
PDB entry 5gkk coloured by chain, front view.
PDB entry 5gkk coloured by chain, side view.
PDB entry 5gkk coloured by chain, top view.
Source organism: Thermotoga neapolitana
Entry author: Ren J

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180709 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homing endonuclease LAGLIDADG domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 20.39 KDa
Source organism: Thermotoga neapolitana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70PR2 (Residues: 1-162; Coverage: 100%)
Gene name: i-Tna1931b
Sequence domains: LAGLIDADG endonuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 32.203Å b: 113.886Å c: 44.922Å
α: 90° β: 110.41° γ: 90°
R-values:
R R work R free
0.207 0.205 0.248
Expression system: Escherichia coli

Quick links