› 5gkw

X-ray diffraction
2.01Å resolution

crystal structure of SZ529 complex with (R,R)-cyclopentanediol

Released:
DOI: 10.2210/pdb5gkw/pdb
PDB entry 5gkw coloured by chain, front view.
PDB entry 5gkw coloured by chain, side view.
PDB entry 5gkw coloured by chain, top view.
Source organism: Rhodococcus erythropolis
Entry authors: Wu L, Sun ZT, Reetz MT, Zhou JH

Function and Biology Details

Reaction catalysed: 
1,2-epoxymenth-8-ene + H(2)O = menth-8-ene-1,2-diol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195601 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Limonene-1,2-epoxide hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 17.32 KDa
Source organism: Rhodococcus erythropolis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZAG3 (Residues: 2-149; Coverage: 99%)
Gene name: limA
Sequence domains: Limonene-1,2-epoxide hydrolase catalytic domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand 6VV 1 x 6VV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P21212
Unit cell:
a: 51.856Å b: 60.541Å c: 119.272Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.263
Expression system: Escherichia coli BL21(DE3)

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