› 5gms

X-ray diffraction
1.7Å resolution

Crystal structure of the mutant S202W/I203F of the esterase E40

Released:
DOI: 10.2210/pdb5gms/pdb
PDB entry 5gms coloured by chain, front view.
PDB entry 5gms coloured by chain, side view.
PDB entry 5gms coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Structural and Mechanistic Insights into the Improvement of the Halotolerance of a Marine Microbial Esterase by Increasing Intra- and Interdomain Hydrophobic Interactions.
Li PY, Zhang Y, Xie BB, Zhang YQ, Hao J, Wang Y, Wang P, Li CY, Qin QL, Zhang XY, Su HN, Shi M, Zhang YZ, Chen XL
Appl Environ Microbiol 83 (2017)
PMID: 28733281

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101292 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 34.07 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0F6WGE1 (Residues: 1-297; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2221
Unit cell:
a: 90.108Å b: 144.845Å c: 96.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Role of gut symbionts of insect pests: A novel target for insect-pest control.
Rupawate et al. (2023)
5 other articles

1 mention without citation

Structural and Mechanistic Insights into the Improvement of the Halotolerance of a Marine Microbial Esterase by Increasing Intra- and Interdomain Hydrophobic Interactions.
Li et al. (2017)