› 5gom

X-ray diffraction
2.8Å resolution

Truncated mitofusin-1, transition-like state

Released:
DOI: 10.2210/pdb5gom/pdb
PDB entry 5gom coloured by chain, front view.
PDB entry 5gom coloured by chain, side view.
PDB entry 5gom coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
MFN1 structures reveal nucleotide-triggered dimerization critical for mitochondrial fusion.
Cao YL, Meng S, Chen Y, Feng JX, Gu DD, Yu B, Li YJ, Yang JY, Liao S, Chan DC, Gao S
OpenAccess logo Nature 542 372-376 (2017)
PMID: 28114303

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184886 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitofusin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 47.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IWA4 (Residues: 1-362, 371-741; Coverage: 55%)
  • Best match: Q8IWA4-2 (Residues: 1-362)
Gene name: MFN1
Sequence domains: Dynamin family

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 146.108Å b: 45.982Å c: 146.18Å
α: 90° β: 92.23° γ: 90°
R-values:
R R work R free
0.193 0.189 0.259
Expression system: Escherichia coli

Quick links

Citations

65 review citations

Mitochondrial dynamics: overview of molecular mechanisms.
Tilokani et al. (2018)
64 more

14 mentions without citation

Mitochondrial dynamics: The dynamin superfamily and execution by collusion.
Ramachandran R. (2018)
13 more