› 5gou

X-ray diffraction
2.91Å resolution

Structure of a 16-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign

Released:
DOI: 10.2210/pdb5gou/pdb
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PDB entry 5gou coloured by chain, front view.
PDB entry 5gou coloured by chain, side view.
PDB entry 5gou coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conversion of the Native 24-mer Ferritin Nanocage into Its Non-Native 16-mer Analogue by Insertion of Extra Amino Acid Residues.
Zhang S, Zang J, Wang W, Chen H, Zhang X, Wang F, Wang H, Zhao G
Angew Chem Int Ed Engl 55 16064-16070 (2016)
PMID: 27885765

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin heavy chain Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 190 amino acids
Theoretical weight: 22.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02794 (Residues: 1-139, 140-183; Coverage: 100%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 99.588Å b: 95.46Å c: 111.973Å
α: 67.08° β: 68.29° γ: 70.06°
R-values:
R R work R free
0.23 0.228 0.275
Expression system: Escherichia coli

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Citations

4 review citations

Protein Assembly by Design.
Zhu et al. (2021)
3 more

PDB-REDO

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