› 5grs

Electron Microscopy
5.4Å resolution

Complex structure of the fission yeast SREBP-SCAP binding domains

Released:
DOI: 10.2210/pdb5grs/pdb
PDB entry 5grs coloured by chain, front view.
PDB entry 5grs coloured by chain, side view.
PDB entry 5grs coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization.
Gong X, Qian H, Shao W, Li J, Wu J, Liu JJ, Li W, Wang HW, Espenshade P, Yan N
OpenAccess logo Cell Res 26 1197-1211 (2016)
PMID: 27811944
Related structures: EMD-9537

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128824 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sterol regulatory element-binding protein cleavage-activating protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 396 amino acids
Theoretical weight: 44.96 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O43043 (Residues: 567-961; Coverage: 36%)
Gene names: SPBC3B9.15c, scp1
Sterol regulatory element-binding protein cleavage-activating protein Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 103 amino acids
Theoretical weight: 11.87 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O43043 (Residues: 986-1085; Coverage: 9%)
Gene names: SPBC3B9.15c, scp1
Sterol regulatory element-binding protein 1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 272 amino acids
Theoretical weight: 30.32 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UUD1 (Residues: 628-896; Coverage: 30%)
Gene names: SPBC19C2.09, sre1
Sequence domains: Domain of unknown function (DUF2014)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.4Å
Relevant EMDB volumes: EMD-9537
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Mechanisms and regulation of cholesterol homeostasis.
Luo et al. (2020)
6 more

1 mention without citation

Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization.
Gong et al. (2016)