› 5gua

X-ray diffraction
1.5Å resolution

Structure of biotin carboxyl carrier protein from pyrococcus horikoshi OT3 (delta N79) A138Y mutant

Released:
DOI: 10.2210/pdb5gua/pdb
PDB entry 5gua coloured by chain, front view.
PDB entry 5gua coloured by chain, side view.
PDB entry 5gua coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Designing better diffracting crystals of biotin carboxyl carrier protein from Pyrococcus horikoshii by a mutation based on the crystal-packing propensity of amino acids.
Yamada KD, Kunishima N, Matsuura Y, Nakai K, Naitow H, Fukasawa Y, Tomii K
OpenAccess logo Acta Crystallogr D Struct Biol 73 757-766 (2017)
PMID: 28876239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipoyl-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 7.79 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O59021 (Residues: 80-149; Coverage: 47%)
Gene name: PH1284
Sequence domains: Biotin-requiring enzyme
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P3121
Unit cell:
a: 39.063Å b: 39.063Å c: 76.642Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.185 0.204
Expression system: Escherichia coli BL21(DE3)

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Citations

1 mention without citation

Designing better diffracting crystals of biotin carboxyl carrier protein from Pyrococcus horikoshii by a mutation based on the crystal-packing propensity of amino acids.
Yamada et al. (2017)