› 5h0o

X-ray diffraction
1.53Å resolution

Crystal structure of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease with manganese ion

Released:
DOI: 10.2210/pdb5h0o/pdb
PDB entry 5h0o coloured by chain, front view.
PDB entry 5h0o coloured by chain, side view.
PDB entry 5h0o coloured by chain, top view.
Source organism: Geobacillus virus E2
Primary publication:
Structural and functional characterization of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease.
Zhang L, Xu D, Huang Y, Zhu X, Rui M, Wan T, Zheng X, Shen Y, Chen X, Ma K, Gong Y
OpenAccess logo Sci Rep 7 42542 (2017)
PMID: 28211904

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-496071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HNH nuclease domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 15.75 KDa
Source organism: Geobacillus virus E2
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1U7Q1S7 (Residues: 1-130; Coverage: 100%)
Sequence domains: HNH endonuclease

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P43212
Unit cell:
a: 66.847Å b: 66.847Å c: 51.511Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.14 0.174
Expression system: Escherichia coli

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Citations

15 citation in other articles

EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation.
Silva et al. (2018)
14 more

3 mentions without citation

Structural and functional characterization of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease.
Zhang et al. (2017)
2 more