› 5h6l

X-ray diffraction
2.1Å resolution

DNA targeting ADP-ribosyltransferase Pierisin-1 in complex with beta-NAD+

Released:
DOI: 10.2210/pdb5h6l/pdb
PDB entry 5h6l coloured by chain, front view.
PDB entry 5h6l coloured by chain, side view.
PDB entry 5h6l coloured by chain, top view.
Source organism: Pieris rapae
Primary publication:
Structural basis of autoinhibition and activation of the DNA-targeting ADP-ribosyltransferase pierisin-1.
Oda T, Hirabayashi H, Shikauchi G, Takamura R, Hiraga K, Minami H, Hashimoto H, Yamamoto M, Wakabayashi K, Shimizu T, Sato M
J Biol Chem 292 15445-15455 (2017)
PMID: 28765284

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pierisin Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 31.23 KDa
Source organism: Pieris rapae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9U8Q4 (Residues: 1-233; Coverage: 27%)
Sequence domains: Scabin-like
Structure domains: Heat-Labile Enterotoxin, subunit A

Ligands and Environments

2 bound ligands:
Ligand NAD 2 x NAD
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P3221
Unit cell:
a: 134.744Å b: 134.744Å c: 73.388Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.243 0.259
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

ADP-ribosylation systems in bacteria and viruses.
Mikolčević et al. (2021)
2 more

1 mention without citation

Structural basis of autoinhibition and activation of the DNA-targeting ADP-ribosyltransferase pierisin-1.
Oda et al. (2017)