› 5h6z

X-ray diffraction
2Å resolution

Crystal structure of Set7, a novel histone methyltransferase in Schizossacharomyces pombe

Released:
DOI: 10.2210/pdb5h6z/pdb
PDB entry 5h6z coloured by chain, front view.
PDB entry 5h6z coloured by chain, side view.
PDB entry 5h6z coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Set7 Is a H3K37 Methyltransferase in Schizosaccharomyces pombe and Is Required for Proper Gametogenesis.
Shen Y, Mevius DEHF, Caliandro R, Carrozzini B, Roh Y, Kim J, Kim S, Ha SC, Morishita M, di Luccio E
Structure 27 631-638.e8 (2019)
PMID: 30773398

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase, H3 lysine-37 specific Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 19.6 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y7Q6 (Residues: 1-147; Coverage: 100%)
Gene names: SPCC297.04c, set7
Sequence domains: SET domain

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand NH4 7 x NH4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P6522
Unit cell:
a: 66.181Å b: 66.181Å c: 257.699Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.172 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

High Risk-Human Papillomavirus in HNSCC: Present and Future Challenges for Epigenetic Therapies.
Ghiani et al. (2022)
1 more