› 5h75

X-ray diffraction
2.74Å resolution

Crystal structure of the MrsD-Protein A fusion protein

Released:
DOI: 10.2210/pdb5h75/pdb
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PDB entry 5h75 coloured by chain, front view.
PDB entry 5h75 coloured by chain, side view.
PDB entry 5h75 coloured by chain, top view.
Source organisms:
Primary publication:
Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.
Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO
OpenAccess logo Sci Rep 7 2595 (2017)
PMID: 28572639

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153666 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein A; Mersacidin decarboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 238 amino acids
Theoretical weight: 26.27 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RC23 (Residues: 1-181, 183-184; Coverage: 94%)
  • Canonical: P38507 (Residues: 220-220, 223-269; Coverage: 10%)
Gene names: mrsD, spa
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 4 x FAD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P213
Unit cell:
a: 167.729Å b: 167.729Å c: 167.729Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.243
Expression system: Escherichia coli

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Citations

1 review citation

Development of imaging scaffolds for cryo-electron microscopy.
Yeates et al. (2020)
11 other articles

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