› 5h83

X-ray diffraction
2.25Å resolution

HETEROYOHIMBINE SYNTHASE HYS FROM CATHARANTHUS ROSEUS - APO FORM

Released:
DOI: 10.2210/pdb5h83/pdb
PDB entry 5h83 coloured by chain, front view.
PDB entry 5h83 coloured by chain, side view.
PDB entry 5h83 coloured by chain, top view.
Source organism: Catharanthus roseus
Primary publication:
Structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity.
Stavrinides A, Tatsis EC, Caputi L, Foureau E, Stevenson CE, Lawson DM, Courdavault V, O'Connor SE
OpenAccess logo Nat Commun 7 12116 (2016)
PMID: 27418042

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heteroyohimbine synthase HYS Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 38.68 KDa
Source organism: Catharanthus roseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1C7D195 (Residues: 1-360; Coverage: 100%)
Gene name: Cr_032583b
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I4
Unit cell:
a: 188.63Å b: 188.63Å c: 58.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Transcriptional Regulation and Transport of Terpenoid Indole Alkaloid in Catharanthus roseus: Exploration of New Research Directions.
Liu et al. (2016)
3 more

2 mentions without citation

Structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity.
Stavrinides et al. (2016)
1 more