› 5hd8

X-ray diffraction
3.15Å resolution

Crystal structure of disulfide cross-linked D417C ClC-ec1

Released:
DOI: 10.2210/pdb5hd8/pdb
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PDB entry 5hd8 coloured by chain, front view.
PDB entry 5hd8 coloured by chain, side view.
PDB entry 5hd8 coloured by chain, top view.
Source organisms:
Entry authors: Mathews II, Khantwal CM, Maduke M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229952 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 450 amino acids
Theoretical weight: 48.03 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 16-465; Coverage: 95%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
FAB FRAGMENT (HEAVY CHAIN) Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
FAB FRAGMENT (LIGHT CHAIN) Chains: D, F

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 231.74Å b: 96.06Å c: 169.95Å
α: 90° β: 131.58° γ: 90°
R-values:
R R work R free
0.208 0.205 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Mus musculus

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