› 5hlb

X-ray diffraction
2.42Å resolution

E. coli PBP1b in complex with acyl-aztreonam and moenomycin

Released:
DOI: 10.2210/pdb5hlb/pdb
PDB entry 5hlb coloured by chain, front view.
PDB entry 5hlb coloured by chain, side view.
PDB entry 5hlb coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Insights into Inhibition of Escherichia coli Penicillin-binding Protein 1B.
King DT, Wasney GA, Nosella M, Fong A, Strynadka NC
J Biol Chem 292 979-993 (2017)
PMID: 27899450

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein 1B Chain: A
Molecule details ›
Chain: A
Length: 747 amino acids
Theoretical weight: 83.28 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02919 (Residues: 58-804; Coverage: 89%)
Gene names: JW0145, b0149, mrcB, pbpF, ponB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand M0E 1 x M0E
Ligand AZR 1 x AZR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Unit cell:
a: 62.53Å b: 64.39Å c: 301.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.225 0.243
Expression system: Escherichia coli

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Citations

1 review citation

Regulation of peptidoglycan synthesis and remodelling.
Egan et al. (2020)
17 other articles

9 mentions without citation

Conserved mechanism of cell-wall synthase regulation revealed by the identification of a new PBP activator in Pseudomonas aeruginosa.
Greene et al. (2018)
8 more