› 5hno

X-ray diffraction
1.7Å resolution

The structure of the kdo-capped saccharide binding subunit of the O-12 specific ABC transporter, Wzt

Released:
DOI: 10.2210/pdb5hno/pdb
PDB entry 5hno coloured by chain, front view.
PDB entry 5hno coloured by chain, side view.
PDB entry 5hno coloured by chain, top view.
Source organism: Raoultella terrigena
Primary publication:
The Klebsiella pneumoniae O12 ATP-binding Cassette (ABC) Transporter Recognizes the Terminal Residue of Its O-antigen Polysaccharide Substrate.
Mann E, Mallette E, Clarke BR, Kimber MS, Whitfield C
J Biol Chem 291 9748-61 (2016)
PMID: 26934919

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-564588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transporter domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 190 amino acids
Theoretical weight: 20.86 KDa
Source organism: Raoultella terrigena
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6U8B1 (Residues: 268-442; Coverage: 40%)
Gene name: wzt
Sequence domains: Wzt C-terminal domain
Structure domains: abc- transporter (atp binding component) like domain

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: C2
Unit cell:
a: 141.75Å b: 53.55Å c: 87.47Å
α: 90° β: 125.77° γ: 90°
R-values:
R R work R free
0.185 0.183 0.216
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure and genetics of Escherichia coli O antigens.
Liu et al. (2020)
2 more

5 mentions without citation

Architecture of a channel-forming O-antigen polysaccharide ABC transporter.
Bi et al. (2018)
4 more