› 5hoi

X-ray diffraction
3.3Å resolution

Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to Tof2.

Released:
DOI: 10.2210/pdb5hoi/pdb
PDB entry 5hoi coloured by chain, front view.
PDB entry 5hoi coloured by chain, side view.
PDB entry 5hoi coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase.
Villa F, Simon AC, Ortiz Bazan MA, Kilkenny ML, Wirthensohn D, Wightman M, Matak-Vinkovíc D, Pellegrini L, Labib K
OpenAccess logo Mol Cell 63 385-96 (2016)
PMID: 27397685

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169558 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase alpha-binding protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 478 amino acids
Theoretical weight: 54.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01454 (Residues: 472-927; Coverage: 49%)
Gene names: CHL15, CTF4, P9659.7, POB1, YPR135W
Sequence domains:
Topoisomerase 1-associated factor 2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 21 amino acids
Theoretical weight: 2.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: Q02208 (Residues: 497-517; Coverage: 3%)
Gene names: TOF2, YK109, YKR010C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Unit cell:
a: 88.576Å b: 99.546Å c: 218.648Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.224
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

31 review citations

Eukaryotic DNA Replication Fork.
Burgers et al. (2017)
30 more

2 mentions without citation

Molecular basis for the production of cyclic peptides by plant asparaginyl endopeptidases.
Jackson et al. (2018)
1 more