Function and Biology Details
Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158840 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NEDD4-like E3 ubiquitin-protein ligase WWP1
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 45.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: HECT-domain (ubiquitin-transferase)
Length: 383 amino acids
Theoretical weight: 45.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q9H0M0 (Residues: 537-917; Coverage: 41%)
Sequence domains: HECT-domain (ubiquitin-transferase)
Ubiquitin
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ubiquitin family
Length: 83 amino acids
Theoretical weight: 9.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P62987 (Residues: 1-73; Coverage: 57%)
Sequence domains: Ubiquitin family
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
APS BEAMLINE 24-ID-C
Spacegroup:
C2
Expression system: Escherichia coli BL21(DE3)
