› 5hqe

X-ray diffraction
1.62Å resolution

Crystal structure of human dihydroorotate dehydrogenase (DHODH) with compound 18T

Released:
DOI: 10.2210/pdb5hqe/pdb
PDB entry 5hqe coloured by chain, front view.
PDB entry 5hqe coloured by chain, side view.
PDB entry 5hqe coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Huang J, Wu D, Lu Q

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + a quinone = orotate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-554201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (quinone), mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 42.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q02127 (Residues: 29-395; Coverage: 93%)
Gene name: DHODH
Sequence domains: Dihydroorotate dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
4 bound ligands:
Ligand 64B 1 x 64B
Ligand ORO 1 x ORO
Ligand SO4 3 x SO4
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P3221
Unit cell:
a: 90.624Å b: 90.624Å c: 123.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.16 0.182
Expression system: Escherichia coli BL21

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