› 5hs0

X-ray diffraction
2.4Å resolution

Computationally Designed Cyclic Tetramer ank1C4_7

Released:
DOI: 10.2210/pdb5hs0/pdb
PDB entry 5hs0 coloured by chain, front view.
PDB entry 5hs0 coloured by chain, side view.
PDB entry 5hs0 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Computational design of self-assembling cyclic protein homo-oligomers.
Fallas JA, Ueda G, Sheffler W, Nguyen V, McNamara DE, Sankaran B, Pereira JH, Parmeggiani F, Brunette TJ, Cascio D, Yeates TR, Zwart P, Baker D
OpenAccess logo Nat Chem 9 353-360 (2017)
PMID: 28338692

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ankyrin domain protein ank1C4_7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 17.94 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6522
Unit cell:
a: 110.45Å b: 110.45Å c: 182.81Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.
Brosey et al. (2019)
8 more

4 mentions without citation

Creation of artificial protein-protein interactions using α-helices as interfaces.
Yagi et al. (2018)
3 more