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1,2-Propanediol Dehydration in Roseburia inulinivorans: STRUCTURAL BASIS FOR SUBSTRATE AND ENANTIOMER SELECTIVITY.

LaMattina JW, Keul ND, Reitzer P, Kapoor S, Galzerani F, Koch DJ, Gouvea IE, Lanzilotta WN
J Biol Chem 291 15515-26 (2016)
Cited: 13 times
EuropePMC logo PMID: 27252380

Abstract

Glycyl radical enzymes (GREs) represent a diverse superfamily of enzymes that utilize a radical mechanism to catalyze difficult, but often essential, chemical reactions. In this work we present the first biochemical and structural data for a GRE-type diol dehydratase from the organism Roseburia inulinivorans (RiDD). Despite high sequence (48% identity) and structural similarity to the GRE-type glycerol dehydratase from Clostridium butyricum, we demonstrate that the RiDD is in fact a diol dehydratase. In addition, the RiDD will utilize both (S)-1,2-propanediol and (R)-1,2-propanediol as a substrate, with an observed preference for the S enantiomer. Based on the new structural information we developed and successfully tested a hypothesis that explains the functional differences we observe.

Articles - 5i2a mentioned but not cited (1)

  1. 1,2-Propanediol Dehydration in Roseburia inulinivorans: STRUCTURAL BASIS FOR SUBSTRATE AND ENANTIOMER SELECTIVITY. LaMattina JW, Keul ND, Reitzer P, Kapoor S, Galzerani F, Koch DJ, Gouvea IE, Lanzilotta WN. J Biol Chem 291 15515-15526 (2016)


Reviews citing this publication (6)

  1. Bacterial microcompartments. Kerfeld CA, Aussignargues C, Zarzycki J, Cai F, Sutter M. Nat Rev Microbiol 16 277-290 (2018)
  2. Sharing vitamins: Cobamides unveil microbial interactions. Sokolovskaya OM, Shelton AN, Taga ME. Science 369 eaba0165 (2020)
  3. New tricks for the glycyl radical enzyme family. Backman LRF, Funk MA, Dawson CD, Drennan CL. Crit Rev Biochem Mol Biol 52 674-695 (2017)
  4. Metabolic functions of the human gut microbiota: the role of metalloenzymes. Rajakovich LJ, Balskus EP. Nat Prod Rep 36 593-625 (2019)
  5. Glycyl Radical Enzyme-Associated Microcompartments: Redox-Replete Bacterial Organelles. Ferlez B, Sutter M, Kerfeld CA. mBio 10 e02327-18 (2019)
  6. Advances in the World of Bacterial Microcompartments. Stewart AM, Stewart KL, Yeates TO, Bobik TA. Trends Biochem Sci 46 406-416 (2021)

Articles citing this publication (6)

  1. A glycyl radical enzyme enables hydrogen sulfide production by the human intestinal bacterium Bilophila wadsworthia. Peck SC, Denger K, Burrichter A, Irwin SM, Balskus EP, Schleheck D. Proc Natl Acad Sci U S A 116 3171-3176 (2019)
  2. A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline. Levin BJ, Huang YY, Peck SC, Wei Y, Martínez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP. Science 355 eaai8386 (2017)
  3. In Vitro Characterization and Concerted Function of Three Core Enzymes of a Glycyl Radical Enzyme - Associated Bacterial Microcompartment. Zarzycki J, Sutter M, Cortina NS, Erb TJ, Kerfeld CA. Sci Rep 7 42757 (2017)
  4. Genomic reconstruction of short-chain fatty acid production by the human gut microbiota. Frolova MS, Suvorova IA, Iablokov SN, Petrov SN, Rodionov DA. Front Mol Biosci 9 949563 (2022)
  5. Molecular basis for catabolism of the abundant metabolite trans-4-hydroxy-L-proline by a microbial glycyl radical enzyme. Backman LR, Huang YY, Andorfer MC, Gold B, Raines RT, Balskus EP, Drennan CL. Elife 9 e51420 (2020)
  6. Molecular basis of C-S bond cleavage in the glycyl radical enzyme isethionate sulfite-lyase. Dawson CD, Irwin SM, Backman LRF, Le C, Wang JX, Vennelakanti V, Yang Z, Kulik HJ, Drennan CL, Balskus EP. Cell Chem Biol 28 1333-1346.e7 (2021)


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