› 5i30

X-ray diffraction
1.9Å resolution

Crystal structure of the human astrovirus 2 neutralizing monoclonal antibody PL-2 Fab fragment at 1.9 A resolution

Released:
DOI: 10.2210/pdb5i30/pdb
PDB entry 5i30 coloured by chain, front view.
PDB entry 5i30 coloured by chain, side view.
PDB entry 5i30 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
De Novo Sequencing and Resurrection of a Human Astrovirus-Neutralizing Antibody.
Bogdanoff WA, Morgenstern D, Bern M, Ueberheide BM, Sanchez-Fauquier A, DuBois RM
OpenAccess logo ACS Infect Dis 2 313-321 (2016)
PMID: 27213181

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230481 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab PL-2 light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.68 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab PL-2 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2221
Unit cell:
a: 72.22Å b: 170.47Å c: 78.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.221

Quick links

Citations

3 review citations

The Astrovirus Capsid: A Review.
Arias et al. (2017)
2 more

3 mentions without citation

An expanded benchmark for antibody-antigen docking and affinity prediction reveals insights into antibody recognition determinants.
Guest et al. (2021)
2 more