› 5i5g

X-ray diffraction
1.95Å resolution

Crystal Structure of Transketolase mutant-R525L from Pichia Stipitis

Released:
DOI: 10.2210/pdb5i5g/pdb
PDB entry 5i5g coloured by chain, front view.
PDB entry 5i5g coloured by chain, side view.
PDB entry 5i5g coloured by chain, top view.
Source organism: Scheffersomyces stipitis CBS 6054
Primary publication:
Structural and biochemical interrogation on transketolase from Pichia stipitis for new functionality.
Hsu LJ, Hsu NS, Wang YL, Wu CJ, Li TL
Protein Eng Des Sel 29 513-522 (2016)
PMID: 27578891

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transketolase Chain: A
Molecule details ›
Chain: A
Length: 697 amino acids
Theoretical weight: 75.01 KDa
Source organism: Scheffersomyces stipitis CBS 6054
Expression system: Escherichia coli
UniProt:
  • Canonical: P34736 (Residues: 1-677; Coverage: 100%)
Gene names: PICST_67105, TKT, TKT1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand POP 1 x POP
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: C2221
Unit cell:
a: 101.121Å b: 184.543Å c: 98.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.186
Expression system: Escherichia coli

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Citations

1 citations in other articles

The Mesomeric Effect of Thiazolium on non-Kekulé Diradicals in Pichia stipitis Transketolase.
Hsu et al. (2018)