› 5i82

X-ray diffraction
2.35Å resolution

First Crystal Structure of E.coli Based Recombinant Diphtheria Toxin Mutant CRM197

Released:
DOI: 10.2210/pdb5i82/pdb
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PDB entry 5i82 coloured by chain, front view.
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PDB entry 5i82 coloured by chain, top view.
Source organism: Corynebacterium diphtheriae
Primary publication:
Structural and immunological characterization of E. coli derived recombinant CRM197 protein used as carrier in conjugate vaccines.
Mishra RPN, Yadav RSP, Jones C, Nocadello S, Minasov G, Shuvalova LA, Anderson WF, Goel A
OpenAccess logo Biosci Rep 38 (2018)
PMID: 29875175

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diphtheria toxin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 535 amino acids
Theoretical weight: 58.48 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6NK15 (Residues: 26-560; Coverage: 100%)
Gene names: DIP0222, tox
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 69.214Å b: 69.167Å c: 127.882Å
α: 90.09° β: 90.01° γ: 82.02°
R-values:
R R work R free
0.21 0.207 0.25
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Harnessing synthetic biology for advancing RNA therapeutics and vaccine design.
Pfeifer et al. (2023)
1 more

7 mentions without citation

Heparin-binding Peptides as Novel Therapies to Stop SARS-CoV-2 Cellular Entry and Infection.
Tavassoly et al. (2020)
6 more

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