› 5i8c

X-ray diffraction
1.54Å resolution

Crystal Structure of HIV-1 Clade A BG505 Fusion Peptide (residue 512-520) in Complex with Broadly Neutralizing Antibody VRC34.01 Fab

Released:
DOI: 10.2210/pdb5i8c/pdb
PDB entry 5i8c coloured by chain, front view.
PDB entry 5i8c coloured by chain, side view.
PDB entry 5i8c coloured by chain, top view.
Source organisms:
Primary publication:
Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody.
Kong R, Xu K, Zhou T, Acharya P, Lemmin T, Liu K, Ozorowski G, Soto C, Taft JD, Bailer RT, Cale EM, Chen L, Choi CW, Chuang GY, Doria-Rose NA, Druz A, Georgiev IS, Gorman J, Huang J, Joyce MG, Louder MK, Ma X, McKee K, O'Dell S, Pancera M, Yang Y, Blanchard SC, Mothes W, Burton DR, Koff WC, Connors M, Ward AB, Kwong PD, Mascola JR
Science 352 828-33 (2016)
PMID: 27174988

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230358 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
VRC34.01 Fab heavy chain Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.8 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
VRC34.01 Fab light chain Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Transmembrane protein gp41 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 846 Da
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: Q2N0S7 (Residues: 509-517; Coverage: 1%)
Gene name: env

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 63.471Å b: 74.895Å c: 84.462Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.182
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

65 review citations

Recent progress in broadly neutralizing antibodies to HIV.
Sok et al. (2018)
64 more

5 mentions without citation

Fusion peptide of HIV-1 as a site of vulnerability to neutralizing antibody.
Kong et al. (2016)
4 more