› 5i97

X-ray diffraction
2.44Å resolution

Structural analysis and inhibition of TraE from the pKM101 type IV secretion system

Released:
DOI: 10.2210/pdb5i97/pdb
Model geometry
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PDB entry 5i97 coloured by chain, front view.
PDB entry 5i97 coloured by chain, side view.
PDB entry 5i97 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural Analysis and Inhibition of TraE from the pKM101 Type IV Secretion System.
Casu B, Smart J, Hancock MA, Smith M, Sygusch J, Baron C
J Biol Chem 291 23817-23829 (2016)
PMID: 27634044

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial virulence protein VirB8 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 163 amino acids
Theoretical weight: 18.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q17U16 (Residues: 70-232; Coverage: 70%)
Gene names: B634_00049, DNQ45_06125, EIZ93_17615, G3V95_21465, KKS20_p00140, KKS54_p00505, KKS75_p00105, KKV62_p00375, NY836_09010, RCS42_P0027, RCS45_P0026, SAMEA4370330_00063, pEcNDM0_00049, pHKU1_33, pMUR050_041, pN3_023, pec386IL_00125, traE, virB8
Sequence domains: VirB8 protein
Structure domains: VirB8 protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 112.049Å b: 123.362Å c: 109.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.24 0.28
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Type IV secretion in Gram-negative and Gram-positive bacteria.
Grohmann et al. (2018)
11 more

4 mentions without citation

Structural Analysis and Inhibition of TraE from the pKM101 Type IV Secretion System.
Casu et al. (2016)
3 more

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