› 5ihv

X-ray diffraction
1.1Å resolution

Crystal structure of a beta-lactamase from Burkholderia ambifaria

Released:
DOI: 10.2210/pdb5ihv/pdb
PDB entry 5ihv coloured by chain, front view.
PDB entry 5ihv coloured by chain, side view.
PDB entry 5ihv coloured by chain, top view.
Source organism: Burkholderia ambifaria MC40-6
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109471 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 269 amino acids
Theoretical weight: 28.19 KDa
Source organism: Burkholderia ambifaria MC40-6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1YX81 (Residues: 39-299; Coverage: 97%)
Gene name: BamMC406_4487
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 49.26Å b: 63.08Å c: 73.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.136 0.167
Expression system: Escherichia coli BL21(DE3)

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