Function and Biology Details
Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
E3 ubiquitin-protein ligase Midline-1 (preferred)
PDBe Complex ID:
PDB-CPX-127435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Midline-1
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
Sequence domains: TRIM C-terminal subgroup One Signature domain
Length: 66 amino acids
Theoretical weight: 7.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
- Canonical:
O15344 (Residues: 320-379; Coverage: 9%)
Sequence domains: TRIM C-terminal subgroup One Signature domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment:
88%
Refinement method:
simulated annealing
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
