› 5ioc

X-ray diffraction
1.9Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99D at cryogenic temperature

Released:
DOI: 10.2210/pdb5ioc/pdb
PDB entry 5ioc coloured by chain, front view.
PDB entry 5ioc coloured by chain, side view.
PDB entry 5ioc coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry authors: Robinson AC, Schlessman JL, Theodoru A, Garcia-Moreno E B

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.2 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 70%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand THP 1 x THP
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 31.033Å b: 60.708Å c: 38.55Å
α: 90° β: 93.3° γ: 90°
R-values:
R R work R free
0.186 0.184 0.235
Expression system: Escherichia coli BL21(DE3)

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