› 5iuz

X-ray diffraction
2.73Å resolution

STRUCTURE OF P450 2B4 F202W MUTANT (CYMAL-5)

Released:
DOI: 10.2210/pdb5iuz/pdb
PDB entry 5iuz coloured by chain, front view.
PDB entry 5iuz coloured by chain, side view.
PDB entry 5iuz coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Effect of detergent binding on cytochrome P450 2B4 structure as analyzed by X-ray crystallography and deuterium-exchange mass spectrometry.
Shah MB, Jang HH, Wilderman PR, Lee D, Li S, Zhang Q, Stout CD, Halpert JR
Biophys Chem 216 1-8 (2016)
PMID: 27280734

Function and Biology Details

Reaction catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 2B4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 54.48 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00178 (Residues: 20-491; Coverage: 96%)
Gene name: CYP2B4
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand CM5 4 x CM5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P31
Unit cell:
a: 91.61Å b: 91.61Å c: 150.931Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.18 0.248
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Relationship between Hepatitis C Virus Infection and Iron Overload.
Zou et al. (2017)
2 more

1 mention without citation

Effect of detergent binding on cytochrome P450 2B4 structure as analyzed by X-ray crystallography and deuterium-exchange mass spectrometry.
Shah et al. (2016)