› 5ixi

X-ray diffraction
2.57Å resolution

Structure of human JAK1 FERM/SH2 in complex with IFNLR1/IL10RA chimera

Released:
DOI: 10.2210/pdb5ixi/pdb
PDB entry 5ixi coloured by chain, front view.
PDB entry 5ixi coloured by chain, side view.
PDB entry 5ixi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Structural Basis for Class II Cytokine Receptor Recognition by JAK1.
Ferrao R, Wallweber HJ, Ho H, Tam C, Franke Y, Quinn J, Lupardus PJ
Structure 24 897-905 (2016)
PMID: 27133025

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149969 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase JAK1 Chain: A
Molecule details ›
Chain: A
Length: 544 amino acids
Theoretical weight: 63.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23458 (Residues: 35-559; Coverage: 46%)
Gene names: JAK1, JAK1A, JAK1B
Sequence domains:
Interferon lambda receptor 1; Interleukin-10 receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 53 amino acids
Theoretical weight: 6.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8IU57 (Residues: 250-260; Coverage: 2%)
  • Canonical: Q13651 (Residues: 264-303; Coverage: 7%)
Gene names: IFNLR1, IL10R, IL10RA, IL28RA, LICR2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 81.17Å b: 110.42Å c: 94.87Å
α: 90° β: 98.11° γ: 90°
R-values:
R R work R free
0.217 0.213 0.255
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

Mechanisms and consequences of Jak-STAT signaling in the immune system.
Villarino et al. (2017)
11 more

4 mentions without citation

The Thrombopoietin Receptor: Structural Basis of Traffic and Activation by Ligand, Mutations, Agonists, and Mutated Calreticulin.
Varghese et al. (2017)
3 more