› 5iz4

X-ray diffraction
1.75Å resolution

Crystal structure of a putative short-chain dehydrogenase/reductase from Burkholderia xenovorans

Released:
DOI: 10.2210/pdb5iz4/pdb
PDB entry 5iz4 coloured by chain, front view.
PDB entry 5iz4 coloured by chain, side view.
PDB entry 5iz4 coloured by chain, top view.
Source organism: Paraburkholderia xenovorans LB400
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171851 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain dehydrogenase/reductase Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 28.53 KDa
Source organism: Paraburkholderia xenovorans LB400
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13GL4 (Residues: 1-263; Coverage: 100%)
Gene name: Bxe_C0900
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I422
Unit cell:
a: 84.61Å b: 84.61Å c: 135.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.182
Expression system: Escherichia coli BL21(DE3)

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