5j0n

Electron Microscopy
11Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-118829 (preferred)
Entry contents:
4 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (8 distinct):
Integrase Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 356 amino acids
Theoretical weight: 40.37 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03700 (Residues: 1-356; Coverage: 100%)
Gene name: int
Sequence domains:
Integration host factor subunit alpha Chains: I, K
Molecule details ›
Chains: I, K
Length: 96 amino acids
Theoretical weight: 11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6X7 (Residues: 2-97; Coverage: 97%)
Gene names: JW1702, b1712, hid, himA, ihfA
Sequence domains: Bacterial DNA-binding protein
Integration host factor subunit beta Chains: J, L
Molecule details ›
Chains: J, L
Length: 94 amino acids
Theoretical weight: 10.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Y1 (Residues: 1-94; Coverage: 100%)
Gene names: JW0895, b0912, himD, hip, ihfB
Sequence domains: Bacterial DNA-binding protein
Excisionase Chains: M, N, O
Molecule details ›
Chains: M, N, O
Length: 55 amino acids
Theoretical weight: 6.79 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03699 (Residues: 1-55; Coverage: 76%)
Gene name: xis
Sequence domains: Excisionase-like protein
attP(-117 to +79) Chain: A
Molecule details ›
Chain: A
Length: 197 nucleotides
Theoretical weight: 60.79 KDa
attB(-21) to attP(+117) Chain: B
Molecule details ›
Chain: B
Length: 139 nucleotides
Theoretical weight: 42.88 KDa
attB(-19 to +21) Chain: C
Molecule details ›
Chain: C
Length: 41 nucleotides
Theoretical weight: 12.61 KDa
attP(-79) to attB(+19) Chain: D
Molecule details ›
Chain: D
Length: 99 nucleotides
Theoretical weight: 30.55 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 11Å
Relevant EMDB volumes: EMD-3400
Expression system: Escherichia coli