› 5j3d

X-ray diffraction
4.08Å resolution

Crystal structure of human Fab 14N4 in complex with post-fusion RSV F

Released:
DOI: 10.2210/pdb5j3d/pdb
PDB entry 5j3d coloured by chain, front view.
PDB entry 5j3d coloured by chain, side view.
PDB entry 5j3d coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for nonneutralizing antibody competition at antigenic site II of the respiratory syncytial virus fusion protein.
Mousa JJ, Sauer MF, Sevy AM, Finn JA, Bates JT, Alvarado G, King HG, Loerinc LB, Fong RH, Doranz BJ, Correia BE, Kalyuzhniy O, Wen X, Jardetzky TS, Schief WR, Ohi MD, Meiler J, Crowe JE
Proc Natl Acad Sci U S A 113 E6849-E6858 (2016)
PMID: 27791117

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229810 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
14N4 heavy chain Chains: A, C, H
Molecule details ›
Chains: A, C, H
Length: 224 amino acids
Theoretical weight: 23.6 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
14N4 light chain Chains: B, D, L
Molecule details ›
Chains: B, D, L
Length: 218 amino acids
Theoretical weight: 24 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fusion glycoprotein F2 Chains: E, G, J
Molecule details ›
Chains: E, G, J
Length: 73 amino acids
Theoretical weight: 8.3 KDa
Source organism: Respiratory syncytial virus
Expression system: Homo sapiens
UniProt:
  • Canonical: P03420 (Residues: 26-98; Coverage: 13%)
Gene name: F
Fusion glycoprotein F1 Chains: F, I, K
Molecule details ›
Chains: F, I, K
Length: 394 amino acids
Theoretical weight: 43.78 KDa
Source organism: Respiratory syncytial virus
Expression system: Homo sapiens
UniProt:
  • Canonical: P03420 (Residues: 147-513; Coverage: 67%)
Gene name: F
Sequence domains: Fusion glycoprotein F0

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P42212
Unit cell:
a: 235.13Å b: 235.13Å c: 220.142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.257 0.282
Expression system: Homo sapiens

Quick links

Citations

6 review citations

Principles of Broad and Potent Antiviral Human Antibodies: Insights for Vaccine Design.
Crowe JE. (2017)
5 more

3 mentions without citation

Antibody Epitopes of Pneumovirus Fusion Proteins.
Huang et al. (2019)
2 more