› 5j9p

X-ray diffraction
2.85Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209485 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Fab Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Expression system: Mus musculus
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 10.09 KDa
Source organism: Streptomyces lividans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 6 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: I4
Unit cell:
a: 155.695Å b: 155.695Å c: 74.791Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.217 0.266
Expression systems:
  • Mus musculus
  • Not provided

Quick links

Citations

7 review citations

A User's Guide to Cell-Free Protein Synthesis.
Gregorio et al. (2019)
6 more

1 mention without citation

Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression.
Focke et al. (2016)