› 5jcg

X-ray diffraction
2.8Å resolution

Structure of Human Peroxiredoxin 3 as three stacked rings

Released:
DOI: 10.2210/pdb5jcg/pdb
PDB entry 5jcg coloured by chain, front view.
PDB entry 5jcg coloured by chain, side view.
PDB entry 5jcg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of Human Peroxiredoxin 3 Suggest Self-Chaperoning Assembly that Maintains Catalytic State.
Yewdall NA, Venugopal H, Desfosses A, Abrishami V, Yosaatmadja Y, Hampton MB, Gerrard JA, Goldstone DC, Mitra AK, Radjainia M
Structure 24 1120-9 (2016)
PMID: 27238969

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 36-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-542071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin-dependent peroxide reductase, mitochondrial Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 201 amino acids
Theoretical weight: 22.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30048 (Residues: 62-256; Coverage: 76%)
Gene names: AOP1, PRDX3
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: I222
Unit cell:
a: 133.188Å b: 168.73Å c: 221.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Multiple Functions and Regulation of Mammalian Peroxiredoxins.
Rhee et al. (2017)
5 more

9 mentions without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)
8 more