› 5jnf

X-ray diffraction
2.75Å resolution

Crystal structure of the LgrA initiation module excluding the Asub domain: F-A-delta-sub

Released:
DOI: 10.2210/pdb5jnf/pdb
PDB entry 5jnf coloured by chain, front view.
PDB entry 5jnf coloured by chain, side view.
PDB entry 5jnf coloured by chain, top view.
Source organism: Brevibacillus parabrevis
Primary publication:
Manipulation of an existing crystal form unexpectedly results in interwoven packing networks with pseudo-translational symmetry
Reimer JM, Aloise MN, Powell HR, Schmeing TM
Acta Crystallogr. D Biol. Crystallogr. 72 1130-1136 (2016)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Linear gramicidin synthase subunit A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 587 amino acids
Theoretical weight: 66.28 KDa
Source organism: Brevibacillus parabrevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70LM7 (Residues: 2-584; Coverage: 26%)
Gene name: lgrA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FMT 5 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41
Unit cell:
a: 161.311Å b: 161.311Å c: 139.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.247 0.275
Expression system: Escherichia coli

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Citations

1 mention without citation

Manipulation of an existing crystal form unexpectedly results in interwoven packing networks with pseudo-translational symmetry.
Reimer et al. (2016)