› 5jqe

X-ray diffraction
3.16Å resolution

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase-8; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 565 amino acids
Theoretical weight: 63.71 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 15-15, 27-27, 29-387, 390-392; Coverage: 95%)
  • Canonical: Q14790 (Residues: 1-186; Coverage: 39%)
Gene names: CASP8, JW3994, MCH5, b4034, malE
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P64
Unit cell:
a: 130.923Å b: 130.923Å c: 67.956Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.216 0.274
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

41 review citations

Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018.
Galluzzi et al. (2018)
40 more

6 mentions without citation

Assembly of platforms for signal transduction in the new era: dimerization, helical filament assembly, and beyond.
Ha et al. (2020)
5 more