5js9

X-ray diffraction
6.92Å resolution

Uncleaved prefusion optimized gp140 trimer with an engineered 8-residue HR1 turn bound to broadly neutralizing antibodies 8ANC195 and PGT128

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-230353 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (15 distinct):
broadly neutralizing antibody PGT128 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 25.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
broadly neutralizing antibody PGT128 light chain Chain: B
Molecule details ›
Chain: B
Length: 211 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Envelope glycoprotein gp160 Chain: C
Molecule details ›
Chain: C
Length: 480 amino acids
Theoretical weight: 53.91 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 30-508; Coverage: 56%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chain: D
Molecule details ›
Chain: D
Length: 140 amino acids
Theoretical weight: 15.7 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 509-661; Coverage: 16%)
Gene name: env
Sequence domains: Retroviral envelope protein
broadly neutralizing antibody 8ANC195 heavy chain Chain: E
Molecule details ›
Chain: E
Length: 238 amino acids
Theoretical weight: 25.27 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
broadly neutralizing antibody 8ANC195 light chain Chain: F
Molecule details ›
Chain: F
Length: 215 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I23
Unit cell:
a: 261.955Å b: 261.955Å c: 261.955Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.288 0.284 0.322
Expression system: Homo sapiens