5jte

Electron Microscopy
3.6Å resolution

Cryo-EM structure of an ErmBL-stalled ribosome in complex with A-, P-, and E-tRNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 57-mer (preferred)
PDBe Complex ID:
PDB-CPX-249843 (preferred)
Entry contents:
50 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (57 distinct):
Small ribosomal subunit protein uS2 Chain: AB
Molecule details ›
Chain: AB
Length: 240 amino acids
Theoretical weight: 26.65 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 1-240; Coverage: 100%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: AC
Molecule details ›
Chain: AC
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 1-233; Coverage: 100%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: AD
Small ribosomal subunit protein uS5 Chain: AE
Small ribosomal subunit protein bS6 Chain: AF
Small ribosomal subunit protein uS7 Chain: AG
Small ribosomal subunit protein uS8 Chain: AH
Small ribosomal subunit protein uS9 Chain: AI
Small ribosomal subunit protein uS10 Chain: AJ
Small ribosomal subunit protein uS11 Chain: AK
Small ribosomal subunit protein uS12 Chain: AL
Small ribosomal subunit protein uS13 Chain: AM
Small ribosomal subunit protein uS14 Chain: AN
Small ribosomal subunit protein uS15 Chain: AO
Small ribosomal subunit protein bS16 Chain: AP
Small ribosomal subunit protein uS17 Chain: AQ
Small ribosomal subunit protein bS18 Chain: AR
Small ribosomal subunit protein uS19 Chain: AS
Small ribosomal subunit protein bS20 Chain: AT
Small ribosomal subunit protein bS21 Chain: AU
Large ribosomal subunit protein uL2 Chain: BC
Large ribosomal subunit protein uL3 Chain: BD
Large ribosomal subunit protein uL4 Chain: BE
Large ribosomal subunit protein uL5 Chain: BF
Large ribosomal subunit protein uL6 Chain: BG
Large ribosomal subunit protein bL9 Chain: BH
Large ribosomal subunit protein uL11 Chain: BI
Large ribosomal subunit protein uL13 Chain: BJ
Large ribosomal subunit protein uL14 Chain: BK
Large ribosomal subunit protein uL15 Chain: BL
Large ribosomal subunit protein uL16 Chain: BM
Large ribosomal subunit protein bL17 Chain: BN
Large ribosomal subunit protein uL18 Chain: BO
Large ribosomal subunit protein bL19 Chain: BP
Large ribosomal subunit protein bL20 Chain: BQ
Large ribosomal subunit protein bL21 Chain: BR
Large ribosomal subunit protein uL22 Chain: BS
Large ribosomal subunit protein uL23 Chain: BT
Large ribosomal subunit protein uL24 Chain: BU
Large ribosomal subunit protein bL25 Chain: BV
Large ribosomal subunit protein bL27 Chain: BW
Large ribosomal subunit protein bL28 Chain: BX
Large ribosomal subunit protein uL29 Chain: BY
Large ribosomal subunit protein uL30 Chain: BZ
Large ribosomal subunit protein bL32 Chain: B0
Large ribosomal subunit protein bL33 Chain: B1
Large ribosomal subunit protein bL34 Chain: B2
Large ribosomal subunit protein bL35 Chain: B3
Large ribosomal subunit protein bL36A Chain: B4
ErmBL Chain: B5
16S ribosomal RNA Chain: AA
Messenger RNA Chain: AV
A-site Lysine tRNA Lysine Chain: AW
P-site tRNA Aspartate Chain: AX
E-site tRNA Valine Chain: AY
23S ribosomal RNA Chain: BA
5S ribosomal RNA Chain: BB

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-8175