› 5jy9

X-ray diffraction
2.16Å resolution

An iron-bound structure of the salicylate synthase Irp9

Released:
DOI: 10.2210/pdb5jy9/pdb
PDB entry 5jy9 coloured by chain, front view.
PDB entry 5jy9 coloured by chain, side view.
PDB entry 5jy9 coloured by chain, top view.
Source organism: Yersinia enterocolitica subsp. enterocolitica 8081
Primary publication:
An Open and Shut Case: The Interaction of Magnesium with MST Enzymes.
Meneely KM, Sundlov JA, Gulick AM, Moran GR, Lamb AL
OpenAccess logo J Am Chem Soc 138 9277-93 (2016)
PMID: 27373320

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chorismate-utilising enzyme C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 434 amino acids
Theoretical weight: 48.06 KDa
Source organism: Yersinia enterocolitica subsp. enterocolitica 8081
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A1JTF3 (Residues: 1-434; Coverage: 100%)
Gene names: YE2612, irp9, ybtS
Sequence domains: chorismate binding enzyme
Structure domains: Anthranilate synthase

Ligands and Environments

3 bound ligands:
Ligand FE2 2 x FE2
Ligand ACT 3 x ACT
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 56.392Å b: 145.351Å c: 58.437Å
α: 90° β: 108.02° γ: 90°
R-values:
R R work R free
0.175 0.172 0.235
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Nonribosomal peptides for iron acquisition: pyochelin biosynthesis as a case study.
Ronnebaum et al. (2018)
2 more

1 mention without citation

An Open and Shut Case: The Interaction of Magnesium with MST Enzymes.
Meneely et al. (2016)