› 5k06

X-ray diffraction
2.5Å resolution

Recombinant bovine beta-lactoglobulin with uncleaved N-terminal methionine (rBlgB)

Released:
DOI: 10.2210/pdb5k06/pdb
PDB entry 5k06 coloured by chain, front view.
PDB entry 5k06 coloured by chain, side view.
PDB entry 5k06 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural Characterisation.
Loch JI, Bonarek P, Tworzydło M, Polit A, Hawro B, Łach A, Ludwin E, Lewiński K
OpenAccess logo Mol Biotechnol 58 605-618 (2016)
PMID: 27380951

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactoglobulin Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.43 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02754 (Residues: 17-178; Coverage: 100%)
Gene name: LGB
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand MYR 1 x MYR
Ligand SIN 1 x SIN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P6422
Unit cell:
a: 106.651Å b: 106.651Å c: 59.312Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.213 0.324
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system.
Suzuki et al. (2018)
1 more

1 mention without citation

Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural Characterisation.
Loch et al. (2016)