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Crystal structure of oxidized Shewanella Yellow Enzyme 4 (SYE4) in complex with p-methoxyphenol

X-ray diffraction
1.5Å resolution
PDB entry 5k1m coloured by chain, front view.
PDB entry 5k1m coloured by chain, side view.
PDB entry 5k1m coloured by chain, top view.
Source organism: Shewanella oneidensis MR-1
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 13008.93 Å2
Buried surface area: 856.26 Å2
Dissociation area: 428.13 Å2
Dissociation energy (ΔGdiss): 7.98 kcal/mol
Dissociation entropy (TΔSdiss): 5.14 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-184340

Macromolecules

NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
Chain: A
Length: 355 amino acids
Theoretical weight: 39.13 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8EBV3 (Residues: 1-355; Coverage: 100%)
Gene names: SO_3392, sye4
Pfam: NADH:flavin oxidoreductase / NADH oxidase family
InterPro:
CATH: Aldolase class I
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 5k1m, assembly 1, front view.
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 5k1m, assembly 1, side view.
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 5k1m, assembly 1, top view.
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