› 5k1y

X-ray diffraction
2.97Å resolution

P2(1) Structure of pNOB8 AspA-DNA complex

Released:
DOI: 10.2210/pdb5k1y/pdb
PDB entry 5k1y coloured by chain, front view.
PDB entry 5k1y coloured by chain, side view.
PDB entry 5k1y coloured by chain, top view.
Source organisms:
Entry author: Schumacher M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118889 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH arsR-type domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 92 amino acids
Theoretical weight: 10.6 KDa
Source organism: Sulfolobus sp. NOB8H2
Expression system: Escherichia coli
UniProt:
  • Canonical: O93706 (Residues: 2-93; Coverage: 99%)
Sequence domains: Sugar-specific transcriptional regulator TrmB
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (33-MER) Chain: P
Molecule details ›
Chain: P
Length: 33 nucleotides
Theoretical weight: 10.04 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (33-MER) Chain: N
Molecule details ›
Chain: N
Length: 33 nucleotides
Theoretical weight: 10.18 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 84.366Å b: 72.306Å c: 90.508Å
α: 90° β: 114.72° γ: 90°
R-values:
R R work R free
0.217 0.21 0.28
Expression systems:
  • Escherichia coli
  • Not provided

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