› 5k2f

Electron crystallography
1Å resolution

Structure of NNQQNY from yeast prion Sup35 with cadmium acetate determined by MicroED

Released:
DOI: 10.2210/pdb5k2f/pdb
PDB entry 5k2f coloured by chain, front view.
PDB entry 5k2f coloured by chain, side view.
PDB entry 5k2f coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED.
Sawaya MR, Rodriguez J, Cascio D, Collazo MJ, Shi D, Reyes FE, Hattne J, Gonen T, Eisenberg DS
Proc Natl Acad Sci U S A 113 11232-11236 (2016)
PMID: 27647903
Related structures: EMD-8197

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic peptide chain release factor GTP-binding subunit Chain: A
Molecule details ›
Chain: A
Length: 6 amino acids
Theoretical weight: 780 Da
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P05453 (Residues: 8-13; Coverage: 1%)
Gene names: GST1, PNM2, SAL3, SUF12, SUP2, SUP35, YD9395.05, YDR172W

Ligands and Environments

2 bound ligands:
Ligand CD 1 x CD
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 1Å
Relevant EMDB volumes: EMD-8197
Expression system: Not provided

Quick links

Citations

15 review citations

A new era for understanding amyloid structures and disease.
Iadanza et al. (2018)
14 more

5 mentions without citation

MicroED opens a new era for biological structure determination.
Nannenga et al. (2016)
4 more